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Lab protocols

We have developed a number of protocols for human postmortem brain tissues in our lab and would be delighted to share

  • Western blot of several genes and proteins from our publications
  • RNA and DNA extractions from bulk postmortem brain tissue samples
  • FACS sorting postmortem brain samples using NeuN+/-
  • RNA/DNA extractions from sorted postmortem brain nuclei
  • Immunohistochemistry for astocyte staining (GFAP) and neuronal staining (NeuN, MAP2, TUJIII) in fixed and fresh human brain
  • Golgi-Cox staining of cortex sections
  • ACD RNAscope in fresh frozen human brain
  • Library prep RNAseq in postmortem brain
  • RNA extraction from human milk