These are older resources Natalie wrote during her PhD and postdoc training that many ECRs found helpful.
For early career researchers
- Postdoc bucket list
- Postdoc-ing 101
- International training tips
- To blog or not to blog?
- Networking just means making friends
- Productivity hacks
- Why I don’t worry about competition
Fellowship and grant help
- My talk on how to get an NHMRC Early Career Fellowship (although the schemes have changed much of this still applies for the EL1 NHMRC grants)
- 10 things I wish I knew at the beginning of my career (+ Youtube)
Starting up
- Making the right moves: a guide for new PIs
- Starting a laboratory, Stanford Lecture
- At the Helm: Leading your laboratory (not free but a recommended read)
Lab protocols
We have developed a number of protocols for human postmortem brain tissues in our lab and would be delighted to share
- Western blot of several genes and proteins from our publications
- RNA and DNA extractions from bulk postmortem brain tissue samples
- FACS sorting postmortem brain samples using NeuN+/-
- RNA/DNA extractions from sorted postmortem brain nuclei
- Immunohistochemistry for astocyte staining (GFAP) and neuronal staining (NeuN, MAP2, TUJIII) in fixed and fresh human brain
- Golgi-Cox staining of cortex sections
- ACD RNAscope in fresh frozen human brain
- Library prep RNAseq in postmortem brain
- RNA extraction from human milk
- H&E staining
- Nissl staining
- Magpix analysis
- Laser capture microscope techniques
- Library prep single nucleus isolation and multi-ome sequencing
- Spatial transcriptomics with the 10x Genomics platforms
